Identification of PSD-95 in the Postsynaptic Density Using MiniSOG and EM Tomography

• Main Authors: Xiaobing Chen (Author), Christine Winters (Author), Virginia Crocker (Author), Michael Lazarou (Author), Alioscka A. Sousa (Author), Richard D. Leapman (Author), Thomas S. Reese (Author)
• Format: Book
• Published: Frontiers Media S.A., 2018-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Combining tomography with electron microscopy (EM) produces images at definition sufficient to visualize individual protein molecules or molecular complexes in intact neurons. When freeze-substituted hippocampal cultures in plastic sections are imaged by EM tomography, detailed structures emerging f...