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Thickness of the aqueous boundary layer in stirred microtitre plate permeability assays (PAMPA and Caco-2), based on the Levich equation

The stirring frequency exponent of -1/2 in the theoretical Levich expression appears to apply to PAMPA and Caco-2 assays, where efficient individual-well magnetic stirring ( > 20 RPM) is used.  If a single molecule is used as a stirring calibrant, then scaling suggested by Eq. (5) with microtitre...

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Main Author: Alex Avdeef (Author)
Format: Book
Published: International Association of Physical Chemists (IAPC), 2022-12-01T00:00:00Z.
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100 1 0 |a Alex Avdeef  |e author 
245 0 0 |a Thickness of the aqueous boundary layer in stirred microtitre plate permeability assays (PAMPA and Caco-2), based on the Levich equation 
260 |b International Association of Physical Chemists (IAPC),   |c 2022-12-01T00:00:00Z. 
500 |a 10.5599/admet.1568 
500 |a 1848-7718 
520 |a The stirring frequency exponent of -1/2 in the theoretical Levich expression appears to apply to PAMPA and Caco-2 assays, where efficient individual-well magnetic stirring ( > 20 RPM) is used.  If a single molecule is used as a stirring calibrant, then scaling suggested by Eq. (5) with microtitre plate data may be used.  The error in calculating hABL based on unscaled hABLref can be as high as 30%.  This is of practical importance in PAMPA, and perhaps cellular assays as well. 
546 |a EN 
690 |a Therapeutics. Pharmacology 
690 |a RM1-950 
655 7 |a article  |2 local 
786 0 |n ADMET and DMPK, Vol 10, Iss 4 (2022) 
787 0 |n http://pub.iapchem.org/ojs/index.php/admet/article/view/1568 
787 0 |n https://doaj.org/toc/1848-7718 
856 4 1 |u https://doaj.org/article/f853b0f24d8b46f5a2d96d11652a49eb  |z Connect to this object online. 

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