Genome-wide spatial expression profiling in formalin-fixed tissues

• Main Authors: Eva Gracia Villacampa (Author), Ludvig Larsson (Author), Reza Mirzazadeh (Author), Linda Kvastad (Author), Alma Andersson (Author), Annelie Mollbrink (Author), Georgia Kokaraki (Author), Vanessa Monteil (Author), Niklas Schultz (Author), Karin Sofia Appelberg (Author), Nuria Montserrat (Author), Haibo Zhang (Author), Josef M. Penninger (Author), Wolfgang Miesbach (Author), Ali Mirazimi (Author), Joseph Carlson (Author), Joakim Lundeberg (Author)
• Format: Book
• Published: Elsevier, 2021-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Summary: Formalin-fixed paraffin embedding (FFPE) is the most widespread long-term tissue preservation approach. Here, we report a procedure to perform genome-wide spatial analysis of mRNA in FFPE-fixed tissue sections, using well-established, commercially available methods for imaging and spatial b...