Recovery of Norovirus from lettuce (Lactuca sativa) using an adsorption -elution method with a negatively charged membrane: comparison of two elution buffers / Recuperação de Norovirus a partir de alface (Lactuca sativa) utilizando um método de adsorção-eluição com membrana negativamente carregada - comparação de dois tampões de eluição

• Main Authors: Marcelo Luiz Lima Brandão (Author), Davi de Oliveira Almeida (Author), Victor Augustus Marin (Author), Marize Pereira Miagostovich (Author)
• Format: Book
• Published: Fundação Oswaldo Cruz (FIOCRUZ), 2014-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Phosphate saline buffer (PBS) and glycine buffer (GB) were evaluated as elution buf-fers in an adsorption-elution method using a negatively charged membrane associated with quantitative polymerase chain reaction (qPCR) and semi-nested PCR for detection of Norovirus genogroup II (NoV GII) from lettuc...