Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits

• Main Authors: Sadeq Eivazi (Author), Jafar Majidi (Author), Leili Aghebati Maleki (Author), Jalal Abdolalizadeh (Author), Mehdi Yousefi (Author), Majid Ahmadi (Author), Somayeh Dadashi (Author), Zahra Moradi (Author), Elmira Zolali (Author)
• Format: Book
• Published: Tabriz University of Medical Sciences, 2015-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Purpose: Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. Methods: Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse IgG2b. Sodium citrate buffer (0.1 M, pH: 3.5)...