Optimization of solvent media to solubilize TEV protease using response surface method

• Main Authors: Niloufar Mohammadian (Author), Hossein Mohammadian (Author), Fatemeh Moazen (Author), Mohammad Hosein Pakdel (Author), Ali Jahanian-Najafabadi (Author), Hamid Mir Mohammad Sadeghi (Author)
• Format: Bog
• Udgivet: Wolters Kluwer Medknow Publications, 2020-01-01T00:00:00Z.
• Fag: article
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Background and purpose: Tobacco etch virus (TEV) protease, a 27 KDa protein, consists of the catalytic domain of nuclear inclusion a (NIa) protein produced by Tobacco etch virus. Because of its unique sequence, TEV protease is used for purging fusion tags from proteins. It also has many advantages s...