PML-RARalpha gene detection method optimization for quantitative PCR Otimização do método de detecção do gene PML-RARalfa para PCR quantitativo

• Main Authors: Jaíra Ferreira de Vasconcellos (Author), Raul Antônio Morais Melo (Author), Fárida Coeli Barros Correia Melo (Author), Washington Batista Neves (Author), Éderson Akio Kido (Author)
• Format: Book
• Published: Sociedade Brasileira de Patologia Clínica, 2008-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Hybrid gene PML-RARalpha is the molecular target found in most cases of acute promyelocytic leukemia (APL) and has been used for diagnosis and minimal residual disease studies. The standard molecular technique employed is qualitative reverse transcriptase-polymerase chain reaction (RT-PCR), but with...