Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA
The presence of the cap structure on the 5'-end of in vitro-transcribed (IVT) mRNA determines its translation and stability, underpinning its use in therapeutics. Both enzymatic and co-transcriptional capping may lead to incomplete positioning of the cap on newly synthesized RNA molecules. IVT...
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MDPI AG,
2022-01-01T00:00:00Z.
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