Ribozyme Assays to Quantify the Capping Efficiency of In Vitro-Transcribed mRNA

• Main Authors: Irena Vlatkovic (Author), János Ludwig (Author), Gábor Boros (Author), Gábor Tamás Szabó (Author), Julia Reichert (Author), Maximilian Buff (Author), Markus Baiersdörfer (Author), Jonas Reinholz (Author), Azita Josefine Mahiny (Author), Uğur Şahin (Author), Katalin Karikó (Author)
• Format: Book
• Published: MDPI AG, 2022-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The presence of the cap structure on the 5'-end of in vitro-transcribed (IVT) mRNA determines its translation and stability, underpinning its use in therapeutics. Both enzymatic and co-transcriptional capping may lead to incomplete positioning of the cap on newly synthesized RNA molecules. IVT...