PINE-TREE enables highly efficient genetic modification of human cell lines

• Päätekijät: Carlye Frisch (Tekijä), William W. Kostes (Tekijä), Brooke Galyon (Tekijä), Brycelyn Whitman (Tekijä), Stefan J. Tekel (Tekijä), Kylie Standage-Beier (Tekijä), Gayathri Srinivasan (Tekijä), Xiao Wang (Tekijä), David A. Brafman (Tekijä)
• Aineistotyyppi: Kirja
• Julkaistu: Elsevier, 2023-09-01T00:00:00Z.
• Aiheet: article
• Linkit: Connect to this object online.

Prime editing technologies enable precise genome editing without the caveats of CRISPR nuclease-based methods. Nonetheless, current approaches to identify and isolate prime-edited cell populations are inefficient. Here, we established a fluorescence-based system, prime-induced nucleotide engineering...