PINE-TREE enables highly efficient genetic modification of human cell lines

• Main Authors: Carlye Frisch (Author), William W. Kostes (Author), Brooke Galyon (Author), Brycelyn Whitman (Author), Stefan J. Tekel (Author), Kylie Standage-Beier (Author), Gayathri Srinivasan (Author), Xiao Wang (Author), David A. Brafman (Author)
• Format: Book
• Published: Elsevier, 2023-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Prime editing technologies enable precise genome editing without the caveats of CRISPR nuclease-based methods. Nonetheless, current approaches to identify and isolate prime-edited cell populations are inefficient. Here, we established a fluorescence-based system, prime-induced nucleotide engineering...