PINE-TREE enables highly efficient genetic modification of human cell lines

• Principais autores: Carlye Frisch (Autor), William W. Kostes (Autor), Brooke Galyon (Autor), Brycelyn Whitman (Autor), Stefan J. Tekel (Autor), Kylie Standage-Beier (Autor), Gayathri Srinivasan (Autor), Xiao Wang (Autor), David A. Brafman (Autor)
• Formato: Livro
• Publicado em: Elsevier, 2023-09-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.

Prime editing technologies enable precise genome editing without the caveats of CRISPR nuclease-based methods. Nonetheless, current approaches to identify and isolate prime-edited cell populations are inefficient. Here, we established a fluorescence-based system, prime-induced nucleotide engineering...