PINE-TREE enables highly efficient genetic modification of human cell lines

• Príomhchruthaitheoirí: Carlye Frisch (Údar), William W. Kostes (Údar), Brooke Galyon (Údar), Brycelyn Whitman (Údar), Stefan J. Tekel (Údar), Kylie Standage-Beier (Údar), Gayathri Srinivasan (Údar), Xiao Wang (Údar), David A. Brafman (Údar)
• Formáid: LEABHAR
• Foilsithe / Cruthaithe: Elsevier, 2023-09-01T00:00:00Z.
• Ábhair: article
• Rochtain ar líne: Connect to this object online.

Prime editing technologies enable precise genome editing without the caveats of CRISPR nuclease-based methods. Nonetheless, current approaches to identify and isolate prime-edited cell populations are inefficient. Here, we established a fluorescence-based system, prime-induced nucleotide engineering...