Comparison of simple and rapid extracting methods of free-tags Mycobacterium tuberculosis protein 64 Recombinant Protein from polyacrylamide gel: Electroelution and the optimized passive elution

• Principais autores: Sri Agung Fitri Kusuma (Autor), Ida Parwati (Autor), Toto Subroto (Autor), Yaya Rukayadi (Autor), Muhammad Fadhlillah (Autor), Asep Rizaludin (Autor)
• Formato: Livro
• Publicado em: Wolters Kluwer Medknow Publications, 2021-01-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.

In this study, the Mycobacterium tuberculosis protein 64 (MPT64) protein was constructed without any tags to facilitate the purification using column affinity chromatography, but the MPT64 must be obtained as a pure protein. This study was purpose to ensure the efficient extracting method to purify...