Contraction of gut smooth muscle cells assessed by fluorescence imaging

• Main Authors: Yohei Tokita (Author), Hirotada Akiho (Author), Kazuhiko Nakamura (Author), Eikichi Ihara (Author), Masahiro Yamamoto (Author)
• Format: Book
• Published: Elsevier, 2015-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Here we discuss the development of a novel cell imaging system for the evaluation of smooth muscle cell (SMC) contraction. SMCs were isolated from the circular and longitudinal muscular layers of mouse small intestine by enzymatic digestion. SMCs were stimulated by test agents, thereafter fixed in a...