A novel hyaluronidase from brown spider (Loxosceles intermedia) venom (Dietrich's Hyaluronidase): from cloning to functional characterization.

• Main Authors: Valéria Pereira Ferrer (Author), Thiago Lopes de Mari (Author), Luiza Helena Gremski (Author), Dilza Trevisan Silva (Author), Rafael Bertoni da Silveira (Author), Waldemiro Gremski (Author), Olga Meiri Chaim (Author), Andrea Senff-Ribeiro (Author), Helena Bonciani Nader (Author), Silvio Sanches Veiga (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2013-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Loxoscelism is the designation given to clinical symptoms evoked by Loxosceles spider's bites. Clinical manifestations include skin necrosis with gravitational spreading and systemic disturbs. The venom contains several enzymatic toxins. Herein, we describe the cloning, expression, refolding an...