Cloning, expression and purification of the α-carbonic anhydrase from the mantle of the Mediterranean mussel, Mytilus galloprovincialis

• Main Authors: Rosa Perfetto (Author), Sonia Del Prete (Author), Daniela Vullo (Author), Vincenzo Carginale (Author), Giovanni Sansone (Author), Carmela M. A. Barone (Author), Mosè Rossi (Author), Fatmah A. S. Alasmary (Author), Sameh M. Osman (Author), Zeid AlOthman (Author), Claudiu T. Supuran (Author), Clemente Capasso (Author)
• Format: Book
• Published: Taylor & Francis Group, 2017-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

We cloned, expressed, purified, and determined the kinetic constants of the recombinant α-carbonic anhydrase (rec-MgaCA) identified in the mantle tissue of the bivalve Mediterranean mussel, Mytilus galloprovincialis. In metazoans, the α-CA family is largely represented and plays a pivotal role in th...