Precise detection of CRISPR-Cas9 editing in hair cells in the treatment of autosomal dominant hearing loss

• Main Authors: Chong Cui (Author), Daqi Wang (Author), Bowei Huang (Author), Fang Wang (Author), Yuxin Chen (Author), Jun Lv (Author), Luping Zhang (Author), Lei Han (Author), Dong Liu (Author), Zheng-Yi Chen (Author), Geng-Lin Li (Author), Huawei Li (Author), Yilai Shu (Author)
• Format: Book
• Published: Elsevier, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Gene therapy would benefit from the effective editing of targeted cells with CRISPR-Cas9 tools. However, it is difficult to precisely assess the editing performance in vivo because the tissues contain many non-targeted cells, which is one of the major barriers to clinical translation. Here, in the A...