Selection of target-binding proteins from the information of weakly enriched phage display libraries by deep sequencing and machine learning

• Principais autores: Tomoyuki Ito (Autor), Thuy Duong Nguyen (Autor), Yutaka Saito (Autor), Yoichi Kurumida (Autor), Hikaru Nakazawa (Autor), Sakiya Kawada (Autor), Hafumi Nishi (Autor), Koji Tsuda (Autor), Tomoshi Kameda (Autor), Mitsuo Umetsu (Autor)
• Formato: Livro
• Publicado em: Taylor & Francis Group, 2023-12-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.

ABSTRACTDespite the advances in surface-display systems for directed evolution, variants with high affinity are not always enriched due to undesirable biases that increase target-unrelated variants during biopanning. Here, our goal was to design a library containing improved variants from the inform...