Selection of target-binding proteins from the information of weakly enriched phage display libraries by deep sequencing and machine learning

• Main Authors: Tomoyuki Ito (Author), Thuy Duong Nguyen (Author), Yutaka Saito (Author), Yoichi Kurumida (Author), Hikaru Nakazawa (Author), Sakiya Kawada (Author), Hafumi Nishi (Author), Koji Tsuda (Author), Tomoshi Kameda (Author), Mitsuo Umetsu (Author)
• Format: Book
• Published: Taylor & Francis Group, 2023-12-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

ABSTRACTDespite the advances in surface-display systems for directed evolution, variants with high affinity are not always enriched due to undesirable biases that increase target-unrelated variants during biopanning. Here, our goal was to design a library containing improved variants from the inform...