Identification of critical chemical modifications and paratope mapping by size exclusion chromatography of stressed antibody-target complexes

• Main Authors: Pavel V. Bondarenko (Author), Andrew C. Nichols (Author), Gang Xiao (Author), Rachel Liuqing Shi (Author), Pik Kay Chan (Author), Thomas M Dillon (Author), Fernando Garces (Author), David J. Semin (Author), Margaret S. Ricci (Author)
• Format: Book
• Published: Taylor & Francis Group, 2021-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Therapeutic proteins including antibodies and Fc-fusion proteins undergo a large number of chemical modifications during cell culture, purification, storage and in human circulation. They are also exposed to harsh conditions during stress studies, including elevated temperature, extremes of pH, forc...