Identification of critical chemical modifications and paratope mapping by size exclusion chromatography of stressed antibody-target complexes

• Главные авторы: Pavel V. Bondarenko (Автор), Andrew C. Nichols (Автор), Gang Xiao (Автор), Rachel Liuqing Shi (Автор), Pik Kay Chan (Автор), Thomas M Dillon (Автор), Fernando Garces (Автор), David J. Semin (Автор), Margaret S. Ricci (Автор)
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• Опубликовано: Taylor & Francis Group, 2021-01-01T00:00:00Z.
• Предметы: article
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Therapeutic proteins including antibodies and Fc-fusion proteins undergo a large number of chemical modifications during cell culture, purification, storage and in human circulation. They are also exposed to harsh conditions during stress studies, including elevated temperature, extremes of pH, forc...