Discovery and identification of EIF2AK2 as a direct key target of berberine for anti-inflammatory effects

• Main Authors: Wei Wei (Author), Qingxuan Zeng (Author), Yan Wang (Author), Xixi Guo (Author), Tianyun Fan (Author), Yinghong Li (Author), Hongbin Deng (Author), Liping Zhao (Author), Xintong Zhang (Author), Yonghua Liu (Author), Yulong Shi (Author), Jingyang Zhu (Author), Xican Ma (Author), Yanxiang Wang (Author), Jiandong Jiang (Author), Danqing Song (Author)
• Format: Book
• Published: Elsevier, 2023-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Using chemoproteomic techniques, we first identified EIF2AK2, eEF1A1, PRDX3 and VPS4B as direct targets of berberine (BBR) for its synergistically anti-inflammatory effects. Of them, BBR has the strongest affinity with EIF2AK2 via two ionic bonds, and regulates several key inflammatory pathways thro...