In Vitro PIG-A Gene Mutation Assay in Human B-Lymphoblastoid TK6 Cells

• Main Authors: Chang-Hui Zhou (Author), Chun-Rong Yu (Author), Peng-Cheng Huang (Author), Ruo-Wan Li (Author), Jing-Ting Wang (Author), Tian-Tian Zhao (Author), Ze-Hao Zhao (Author), Jing Ma (Author), Yan Chang (Author)
• Format: Book
• Published: Georg Thieme Verlag KG, 2021-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The X-linked PIG-A gene is involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. PIG-A mutant cells fail to synthesize GPI and to express GPI-anchored protein markers (e.g., CD59 and CD55). In recent years, in vitro PIG-A assay has been established based on the high conservatio...