Targeted Disruption of V600E-Mutant BRAF Gene by CRISPR-Cpf1

• Main Authors: Meijia Yang (Author), Heng Wei (Author), Yuelong Wang (Author), Jiaojiao Deng (Author), Yani Tang (Author), Liangxue Zhou (Author), Gang Guo (Author), Aiping Tong (Author)
• Format: Book
• Published: Elsevier, 2017-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

BRAF-V600E (1799T > A) is one of the most frequently reported driver mutations in multiple types of cancers, and patients with such mutations could benefit from selectively inactivating the mutant allele. Near this mutation site, there are two TTTN and one NGG protospacer-adjacent motifs (PAMs) f...