Combining phage display with SMRTbell next-generation sequencing for the rapid discovery of functional scFv fragments

• Main Authors: Francesco Nannini (Author), Lenart Senicar (Author), Farhaan Parekh (Author), Khai J. Kong (Author), Alexander Kinna (Author), Reyisa Bughda (Author), James Sillibourne (Author), Xihao Hu (Author), Biao Ma (Author), Yuchen Bai (Author), Mathieu Ferrari (Author), Martin A. Pule (Author), Shimobi C. Onuoha (Author)
• Format: Book
• Published: Taylor & Francis Group, 2021-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Phage display technology in combination with next-generation sequencing (NGS) currently is a state-of-the-art method for the enrichment and isolation of monoclonal antibodies from diverse libraries. However, the current NGS methods employed for sequencing phage display libraries are limited by the s...