Lateral flow immunoassay for rapid and sensitive detection of dsRNA contaminants in in vitro-transcribed mRNA products

• Hauptverfasser: Dengwang Luo (Verfasst von), Zhanfeng Wu (Verfasst von), Daming Wang (Verfasst von), Jieli Zhang (Verfasst von), Fei Shao (Verfasst von), Shuo Wang (Verfasst von), Stefano Cestellos-Blanco (Verfasst von), Dawei Xu (Verfasst von), Yuhong Cao (Verfasst von)
• Format: Buch
• Veröffentlicht: Elsevier, 2023-06-01T00:00:00Z.
• Schlagworte: article
• Online-Zugang: Connect to this object online.

High purity is essential in mRNA-based therapeutic applications. A major contaminant of in vitro-transcribed (IVT) mRNA manufacturing is double-stranded RNA (dsRNA), which can induce severe anti-viral immune responses. Detection methods, such as agarose gel electrophoresis, ELISA, and dot-blot assay...