Lateral flow immunoassay for rapid and sensitive detection of dsRNA contaminants in in vitro-transcribed mRNA products

• Main Authors: Dengwang Luo (Author), Zhanfeng Wu (Author), Daming Wang (Author), Jieli Zhang (Author), Fei Shao (Author), Shuo Wang (Author), Stefano Cestellos-Blanco (Author), Dawei Xu (Author), Yuhong Cao (Author)
• Format: Book
• Published: Elsevier, 2023-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

High purity is essential in mRNA-based therapeutic applications. A major contaminant of in vitro-transcribed (IVT) mRNA manufacturing is double-stranded RNA (dsRNA), which can induce severe anti-viral immune responses. Detection methods, such as agarose gel electrophoresis, ELISA, and dot-blot assay...