Bioprocess and downstream optimization of recombinant human growth hormone in Pichia pastoris

• Main Authors: Saeed Azadi (Author), Seyed Kazem Sadjady (Author), Seyed Alireza Mortazavi (Author), Nasser Naghdi (Author), Arash Mahboubi (Author), Roya Solaimanian (Author)
• Format: Book
• Published: Wolters Kluwer Medknow Publications, 2018-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The methylotrophic yeast Pichia pastoris is a well-established expression host, which is often used in the production of protein pharmaceuticals. This work aimed to evaluate the effect of various concentrations of ascorbic acid in mixed feeding strategy with sorbitol/methanol on productivity of reco...