Knockout of ABC transporters by CRISPR/Cas9 contributes to reliable and accurate transporter substrate identification for drug discovery

• Главные авторы: Dongyan Feng (Автор), Guorui Zhong (Автор), Qingxia Zuo (Автор), Yanbin Wan (Автор), Wanqing Xu (Автор), Changsheng He (Автор), Cailing Lin (Автор), Dongchao Huang (Автор), Feng Chen (Автор), Lizhen Huang (Автор)
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• Опубликовано: Frontiers Media S.A., 2022-10-01T00:00:00Z.
• Предметы: article
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It is essential to explore the relationship between drugs and transporters in the process of drug development. Strong background signals in nonhuman MDCK or LLC-PK1 cells and overlapping interference of inhibitors or RNAi in human Caco-2 cells mean that an ideal alternative could be to knock out spe...