Knockout of ABC transporters by CRISPR/Cas9 contributes to reliable and accurate transporter substrate identification for drug discovery

• Main Authors: Dongyan Feng (Author), Guorui Zhong (Author), Qingxia Zuo (Author), Yanbin Wan (Author), Wanqing Xu (Author), Changsheng He (Author), Cailing Lin (Author), Dongchao Huang (Author), Feng Chen (Author), Lizhen Huang (Author)
• Format: Book
• Published: Frontiers Media S.A., 2022-10-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

It is essential to explore the relationship between drugs and transporters in the process of drug development. Strong background signals in nonhuman MDCK or LLC-PK1 cells and overlapping interference of inhibitors or RNAi in human Caco-2 cells mean that an ideal alternative could be to knock out spe...