Introduction of a More Glutaredoxin-like Active Site to PDI Results in Competition between Protein Substrate and Glutathione Binding

• Main Authors: Mirva J. Saaranen (Author), Heli I. Alanen (Author), Kirsi E. H. Salo (Author), Emmanuel Nji (Author), Pekka Kärkkäinen (Author), Constanze Schmotz (Author), Lloyd W. Ruddock (Author)
• Format: Book
• Published: MDPI AG, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Proteins in the thioredoxin superfamily share a similar fold, contain a -CXXC- active site, and catalyze oxidoreductase reactions by dithiol-disulfide exchange mechanisms. Protein disulfide isomerase (PDI) has two -CGHC- active sites. For in vitro studies, oxidation/reduction of PDI during the catal...