Introduction of a More Glutaredoxin-like Active Site to PDI Results in Competition between Protein Substrate and Glutathione Binding

• Autores principales: Mirva J. Saaranen (Autor), Heli I. Alanen (Autor), Kirsi E. H. Salo (Autor), Emmanuel Nji (Autor), Pekka Kärkkäinen (Autor), Constanze Schmotz (Autor), Lloyd W. Ruddock (Autor)
• Formato: Libro
• Publicado: MDPI AG, 2022-09-01T00:00:00Z.
• Materias: article
• Acceso en línea: Connect to this object online.

Proteins in the thioredoxin superfamily share a similar fold, contain a -CXXC- active site, and catalyze oxidoreductase reactions by dithiol-disulfide exchange mechanisms. Protein disulfide isomerase (PDI) has two -CGHC- active sites. For in vitro studies, oxidation/reduction of PDI during the catal...