2D NMR Analysis as a Sensitive Tool for Evaluating the Higher-Order Structural Integrity of Monoclonal Antibody against COVID-19

• Main Authors: Francesca Cantini (Author), Emanuele Andreano (Author), Ida Paciello (Author), Veronica Ghini (Author), Francesco Berti (Author), Rino Rappuoli (Author), Lucia Banci (Author)
• Format: Book
• Published: MDPI AG, 2022-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The higher-order structure (HOS) of protein therapeutics has been confirmed as a critical quality parameter. In this study, we compared 2D ¹H-¹³C ALSOFAST-HMQC NMR spectra with immunochemical ELISA-based analysis to evaluate their sensitivity in assessin...