2D NMR Analysis as a Sensitive Tool for Evaluating the Higher-Order Structural Integrity of Monoclonal Antibody against COVID-19

• Glavni autori: Francesca Cantini (Autor), Emanuele Andreano (Autor), Ida Paciello (Autor), Veronica Ghini (Autor), Francesco Berti (Autor), Rino Rappuoli (Autor), Lucia Banci (Autor)
• Format: Knjiga
• Izdano: MDPI AG, 2022-09-01T00:00:00Z.
• Teme: article
• Online pristup: Connect to this object online.

The higher-order structure (HOS) of protein therapeutics has been confirmed as a critical quality parameter. In this study, we compared 2D ¹H-¹³C ALSOFAST-HMQC NMR spectra with immunochemical ELISA-based analysis to evaluate their sensitivity in assessin...