Diagnosis of brugian filariasis by loop-mediated isothermal amplification.

• المؤلفون الرئيسيون: Catherine B Poole (مؤلف), Nathan A Tanner (مؤلف), Yinhua Zhang (مؤلف), Thomas C Evans (مؤلف), Clotilde K S Carlow (مؤلف)
• التنسيق: كتاب
• منشور في: Public Library of Science (PLoS), 2012-01-01T00:00:00Z.
• الموضوعات: article
• الوصول للمادة أونلاين: Connect to this object online.

In this study we developed and evaluated a Brugia Hha I repeat loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Brugia genomic DNA. Amplification was detected using turbidity or fluorescence as readouts. Reactions generated a turbidity threshold value or a clear visual...