Diagnosis of brugian filariasis by loop-mediated isothermal amplification.

• Main Authors: Catherine B Poole (Author), Nathan A Tanner (Author), Yinhua Zhang (Author), Thomas C Evans (Author), Clotilde K S Carlow (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2012-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In this study we developed and evaluated a Brugia Hha I repeat loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Brugia genomic DNA. Amplification was detected using turbidity or fluorescence as readouts. Reactions generated a turbidity threshold value or a clear visual...