A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish

• Главные авторы: Sergey V. Prykhozhij (Автор), Shelby L. Steele (Автор), Babak Razaghi (Автор), Jason N. Berman (Автор)
• Формат: ‌
• Опубликовано: The Company of Biologists, 2017-06-01T00:00:00Z.
• Предметы: article
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Clustered regularly interspaced palindromic repeats (CRISPR)/Cas-based adaptive immunity against pathogens in bacteria has been adapted for genome editing and applied in zebrafish (Danio rerio) to generate frameshift mutations in protein-coding genes. Although there are methods to detect, quantify a...