A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish
Clustered regularly interspaced palindromic repeats (CRISPR)/Cas-based adaptive immunity against pathogens in bacteria has been adapted for genome editing and applied in zebrafish (Danio rerio) to generate frameshift mutations in protein-coding genes. Although there are methods to detect, quantify a...
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Format: | Book |
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The Company of Biologists,
2017-06-01T00:00:00Z.
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A1234.567 |
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