A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafish

Clustered regularly interspaced palindromic repeats (CRISPR)/Cas-based adaptive immunity against pathogens in bacteria has been adapted for genome editing and applied in zebrafish (Danio rerio) to generate frameshift mutations in protein-coding genes. Although there are methods to detect, quantify a...

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Bibliographic Details
Main Authors: Sergey V. Prykhozhij (Author), Shelby L. Steele (Author), Babak Razaghi (Author), Jason N. Berman (Author)
Format: Book
Published: The Company of Biologists, 2017-06-01T00:00:00Z.
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3rd Floor Main Library

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Call Number: A1234.567
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