Fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse Aedes aegypti strain.

• Main Authors: Alexander W E Franz (Author), Irma Sanchez-Vargas (Author), Robyn R Raban (Author), William C Black (Author), Anthony A James (Author), Ken E Olson (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2014-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In 2006, we reported a mariner (Mos1)-transformed Aedes aegypti line, Carb77, which was highly resistant to dengue-2 virus (DENV2). Carb77 mosquitoes expressed a DENV2-specific inverted-repeat (IR) RNA in midgut epithelial cells after ingesting an infectious bloodmeal. The IR-RNA formed double-stran...