Type I Interferons Impede Short Hairpin RNA-Mediated RNAi via Inhibition of Dicer-Mediated Processing to Small Interfering RNA

• Main Authors: Mitsuhiro Machitani (Author), Fuminori Sakurai (Author), Keisaku Wakabayashi (Author), Kosuke Takayama (Author), Masashi Tachibana (Author), Hiroyuki Mizuguchi (Author)
• Format: Book
• Published: Elsevier, 2017-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

RNAi by short hairpin RNA (shRNA) is a powerful tool not only for studying gene functions in various organisms, including mammals, but also for the treatment of severe disorders. However, shRNA-expressing vectors can induce type I interferon (IFN) expression by activation of innate immune responses,...