LC-MS/MS Method for Measurement of Thiopurine Nucleotides (TN) in Erythrocytes and Association of TN Concentrations With TPMT Enzyme Activity

• Autori principali: Amol O. Bajaj (Autore), Mark M. Kushnir (Autore), Erik Kish-Trier (Autore), Rachel N. Law (Autore), Lauren M. Zuromski (Autore), Alejandro R. Molinelli (Autore), Gwendolyn A. McMillin (Autore), Kamisha L. Johnson-Davis (Autore)
• Natura: Libro
• Pubblicazione: Frontiers Media S.A., 2022-03-01T00:00:00Z.
• Soggetti: article
• Accesso online: Connect to this object online.

Monitoring concentrations of thiopurine metabolites is used clinically to prevent adverse effects in patients on thiopurine drug therapy. We developed a LC-MS/MS method for the quantification of 6-thioguanine (6-TG) and 6-methylmercaptopurine (6-MMP) in red blood cells (RBCs). This method utilizes a...