ECNano: A cost-effective workflow for target enrichment sequencing and accurate variant calling on 4800 clinically significant genes using a single MinION flowcell

• Main Authors: Amy Wing-Sze Leung (Author), Henry Chi-Ming Leung (Author), Chak-Lim Wong (Author), Zhen-Xian Zheng (Author), Wui-Wang Lui (Author), Ho-Ming Luk (Author), Ivan Fai-Man Lo (Author), Ruibang Luo (Author), Tak-Wah Lam (Author)
• Format: Book
• Published: BMC, 2022-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background The application of long-read sequencing using the Oxford Nanopore Technologies (ONT) MinION sequencer is getting more diverse in the medical field. Having a high sequencing error of ONT and limited throughput from a single MinION flowcell, however, limits its applicability for ac...