piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice

• প্রধান লেখক: Rongbo Li (লেখক), Yuan Zhuang (লেখক), Min Han (লেখক), Tian Xu (লেখক), Xiaohui Wu (লেখক)
• বিন্যাস: গ্রন্থ
• প্রকাশিত: The Company of Biologists, 2013-05-01T00:00:00Z.
• বিষয়গুলি: article
• অনলাইন ব্যবহার করুন: Connect to this object online.

SUMMARY The stable genomic integration and expression of a large transgene is a major hurdle in gene therapy. We show that the modified piggyBac (PB) transposon system can be used to introduce a 207 kb genomic DNA fragment containing the RORγ/γt locus into human cells and mice. PB-mediated transgene...