A preparation strategy for protein-oriented immobilized silica magnetic beads with Spy chemistry for ligand fishing

• 主要な著者: Yu Yi (著者), Jianming Hu (著者), Shenwei Ding (著者), Jianfeng Mei (著者), Xudong Wang (著者), Yanlu Zhang (著者), Jianshu Chen (著者), Guoqing Ying (著者)
• フォーマット: 図書
• 出版事項: Elsevier, 2022-06-01T00:00:00Z.
• 主題: article
• オンライン･アクセス: Connect to this object online.

Due to the complexity of bioactive ingredients in biological samples, the screening of target proteins is a complex process. Herein, a feasible strategy for directing protein immobilization on silica magnetic beads for ligand fishing based on SpyTag/SpyCatcher (ST/SC)-mediated anchoring is presented...