A preparation strategy for protein-oriented immobilized silica magnetic beads with Spy chemistry for ligand fishing

• Main Authors: Yu Yi (Author), Jianming Hu (Author), Shenwei Ding (Author), Jianfeng Mei (Author), Xudong Wang (Author), Yanlu Zhang (Author), Jianshu Chen (Author), Guoqing Ying (Author)
• Format: Book
• Published: Elsevier, 2022-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Due to the complexity of bioactive ingredients in biological samples, the screening of target proteins is a complex process. Herein, a feasible strategy for directing protein immobilization on silica magnetic beads for ligand fishing based on SpyTag/SpyCatcher (ST/SC)-mediated anchoring is presented...