A novel base editor SpRY-ABE8eF148A mediates efficient A-to-G base editing with a reduced off-target effect

• প্রধান লেখক: Guo Li (লেখক), Yaxian Cheng (লেখক), Yeqiu Li (লেখক), Hongru Ma (লেখক), Zhongji Pu (লেখক), Sa Li (লেখক), Yiqiang Zhao (লেখক), Xingxu Huang (লেখক), Yuan Yao (লেখক)
• বিন্যাস: গ্রন্থ
• প্রকাশিত: Elsevier, 2023-03-01T00:00:00Z.
• বিষয়গুলি: article
• অনলাইন ব্যবহার করুন: Connect to this object online.

Adenine base editors (ABEs) can mediate two transition mutations, A-to-G and T-to-C, which are suitable for repairing G·C-to-T·A pathogenic variants, the most significant human pathogenic variant. By combining the protospacer adjacent motif (PAM)less SpRY nuclease with F148A-mutated TadA∗8e deaminas...