A novel base editor SpRY-ABE8eF148A mediates efficient A-to-G base editing with a reduced off-target effect

• Main Authors: Guo Li (Author), Yaxian Cheng (Author), Yeqiu Li (Author), Hongru Ma (Author), Zhongji Pu (Author), Sa Li (Author), Yiqiang Zhao (Author), Xingxu Huang (Author), Yuan Yao (Author)
• Format: Book
• Published: Elsevier, 2023-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Adenine base editors (ABEs) can mediate two transition mutations, A-to-G and T-to-C, which are suitable for repairing G·C-to-T·A pathogenic variants, the most significant human pathogenic variant. By combining the protospacer adjacent motif (PAM)less SpRY nuclease with F148A-mutated TadA∗8e deaminas...