A novel base editor SpRY-ABE8eF148A mediates efficient A-to-G base editing with a reduced off-target effect

• Päätekijät: Guo Li (Tekijä), Yaxian Cheng (Tekijä), Yeqiu Li (Tekijä), Hongru Ma (Tekijä), Zhongji Pu (Tekijä), Sa Li (Tekijä), Yiqiang Zhao (Tekijä), Xingxu Huang (Tekijä), Yuan Yao (Tekijä)
• Aineistotyyppi: Kirja
• Julkaistu: Elsevier, 2023-03-01T00:00:00Z.
• Aiheet: article
• Linkit: Connect to this object online.

Adenine base editors (ABEs) can mediate two transition mutations, A-to-G and T-to-C, which are suitable for repairing G·C-to-T·A pathogenic variants, the most significant human pathogenic variant. By combining the protospacer adjacent motif (PAM)less SpRY nuclease with F148A-mutated TadA∗8e deaminas...