Membrane tethering of CreER decreases uninduced cell labeling and cytotoxicity while maintaining recombination efficiency

• Main Authors: Mianqiao Chen (Author), Xiong Tian (Author), Liqun Xu (Author), Ruolan Wu (Author), Haoming He (Author), Haibao Zhu (Author), Wencan Xu (Author), Chi-ju Wei (Author)
• Format: Book
• Published: Elsevier, 2022-03-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Genetic lineage tracing is indispensable to unraveling the origin, fate, and plasticity of cells. However, the intrinsic leakiness in the CreER-loxP system raises concerns on data interpretation. Here, we reported the generation of a novel dual inducible CreER-loxP system with superior labeling char...