All-potassium channel CRISPR screening reveals a lysine-specific pathway of insulin secretion

• Egile Nagusiak: Jing Lu (Egilea), Ru-Xuan Zhao (Egilea), Feng-Ran Xiong (Egilea), Juan-Juan Zhu (Egilea), Ting-Ting Shi (Egilea), Ying-Chao Zhang (Egilea), Gong-Xin Peng (Egilea), Jin-Kui Yang (Egilea)
• Formatua: Liburua
• Argitaratua: Elsevier, 2024-02-01T00:00:00Z.
• Gaiak: article
• Sarrera elektronikoa: Connect to this object online.

Objective: Genome-scale CRISPR-Cas9 knockout coupled with single-cell RNA sequencing (scRNA-seq) has been used to identify function-related genes. However, this method may knock out too many genes, leading to low efficiency in finding genes of interest. Insulin secretion is controlled by several ele...