All-potassium channel CRISPR screening reveals a lysine-specific pathway of insulin secretion

• Hoofdauteurs: Jing Lu (Auteur), Ru-Xuan Zhao (Auteur), Feng-Ran Xiong (Auteur), Juan-Juan Zhu (Auteur), Ting-Ting Shi (Auteur), Ying-Chao Zhang (Auteur), Gong-Xin Peng (Auteur), Jin-Kui Yang (Auteur)
• Formaat: Boek
• Gepubliceerd in: Elsevier, 2024-02-01T00:00:00Z.
• Onderwerpen: article
• Online toegang: Connect to this object online.

Objective: Genome-scale CRISPR-Cas9 knockout coupled with single-cell RNA sequencing (scRNA-seq) has been used to identify function-related genes. However, this method may knock out too many genes, leading to low efficiency in finding genes of interest. Insulin secretion is controlled by several ele...