All-potassium channel CRISPR screening reveals a lysine-specific pathway of insulin secretion

• Main Authors: Jing Lu (Author), Ru-Xuan Zhao (Author), Feng-Ran Xiong (Author), Juan-Juan Zhu (Author), Ting-Ting Shi (Author), Ying-Chao Zhang (Author), Gong-Xin Peng (Author), Jin-Kui Yang (Author)
• Format: Book
• Published: Elsevier, 2024-02-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Objective: Genome-scale CRISPR-Cas9 knockout coupled with single-cell RNA sequencing (scRNA-seq) has been used to identify function-related genes. However, this method may knock out too many genes, leading to low efficiency in finding genes of interest. Insulin secretion is controlled by several ele...